DrOmics Labs

Mid-Throughput Short-Read Sequencing

Unveiling the Potential of Mid-Throughput Short-Read Sequencing

In the ever-evolving landscape of genomic research, mid-throughput short-read sequencing stands as a beacon of efficiency and precision. This powerful technology is revolutionizing our approach to genetic analysis, offering a balance between high-throughput and low-throughput sequencing methods.

What is Mid-Throughput Short-Read Sequencing?

Mid-throughput short-read sequencing is a technique that allows for the rapid and accurate analysis of DNA sequences. It is particularly well-suited for applications that require a moderate amount of data, such as targeted gene panels or small genome sequencing.

The Onso System: A New Era in Short-Read Sequencing

The Onso system by PacBio is a groundbreaking innovation in the field of mid-throughput short-read sequencing. It offers an extraordinary level of accuracy with its sequencing by binding (SBB) technology, delivering ultra-high Q40+ data quality1. This means that researchers can confidently identify rare variants that might be missed by other short-read sequencing platforms.

Advantages of Mid-Throughput Short-Read Sequencing

Enhanced Accuracy

The Onso system’s Q40+ performance provides high-quality results at up to fourfold less read depth than SBS, making it a cost-effective solution for many research applications.


Mid-throughput short-read sequencing can power through difficult-to-sequence regions of the genome, providing researchers with accurate characterization of low complexity, highly repetitive regions.

Integration with Existing Ecosystems

The Onso system seamlessly integrates with existing short-read sequencing ecosystems, supporting a diverse set of popular short-read applications.

Applications of Mid-Throughput Short-Read Sequencing

Cancer Research

Mid-throughput short-read sequencing facilitates the development of screening, monitoring, and therapy selection in cancer research.

Gene Editing Research

It aids in confirmation of potential editing outcomes and discovering biomarkers, crucial for advancing gene editing technologies.


Validated whole-exome sequencing (WES) using hybrid capture protocols can be performed efficiently with mid-throughput sequencing.

Single Cell Analysis

The technology supports and validates libraries for single-cell analysis, expanding the possibilities in this intricate field.


Mid-throughput short-read sequencing is a versatile and accurate tool that bridges the gap between high-throughput and low-throughput sequencing. It is an invaluable asset for researchers aiming to push the boundaries of genetic analysis and unlock new insights into the complex world of genomics.

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